3rd Generation DNA Sequencing
We are about to enter an era of DNA sequencing where one can produce, de novo, a reference-quality genome of any living species for 1,000 EU. This ability will revolutionize ecology, evolution, and conservation science and effectively mark the beginning of a new exploration of the natural world.
In this tutorial we will present the current state of the art for 3rd-gen long read sequencing and assembly and give an overview of the computational problems and challenges involved in interpreting this data.
- E. Myers, ``Efficient Alignment Discovery amongst Noisy Long Reads,'' IProc. Workshop on Algorithms for BioInformatics (WABI, Wroclaw, Poland 2014), 52-67. Also published as Springer-Verlag LNCS #8791.
- M.A. Grohme, S. Schloissnig, A. Rozanski, M. Pippel, G. Young, S. Winkler, H. Brandl, I. Henry, A. Dahl, S. Powell, M. Hiller, E.W. Myers, J.C. Rink, ``The Genome of S. mediterranea and the Evolution of Cellular Core Mechanisms,'' Nature, accepted.
- C.S. Chin, P. Peluso, F.J. Sedlazeck, M. Nattestad, G.T. Concepcion, A. Clum, C. Dunn, R. O'Malley, R. Figueroa-Balderas, A. Morales-Cruz, G.R. Cramer, M. Delledonne, C. Luo, J.R. Ecker, D. Cantu, R.R. Rank, M.C. Schatz, .``Phased diploid genome assembly with single-molecule real-time sequencing, '' Nature Methods 13,12 (2016):1050-1054.